Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
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| Định dạng: | Sách |
| Ngôn ngữ: | English |
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Springer
2023
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| Truy cập trực tuyến: | https://link.springer.com/article/10.1186/s42649-023-00091-8 https://dlib.phenikaa-uni.edu.vn/handle/PNK/9064 |
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oai:localhost:PNK-90642023-09-18T04:10:57Z Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning Ohnishi, Chie Ohnishi, Takashi Ntiamoah, Peter IHC-CS CCS CC-BY In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for calibrating the color variation due to the scanner. 2023-09-18T04:10:57Z 2023-09-18T04:10:57Z 2023 Book https://link.springer.com/article/10.1186/s42649-023-00091-8 https://dlib.phenikaa-uni.edu.vn/handle/PNK/9064 en application/pdf Springer |
| institution |
Digital Phenikaa |
| collection |
Digital Phenikaa |
| language |
English |
| topic |
IHC-CS CCS |
| spellingShingle |
IHC-CS CCS Ohnishi, Chie Ohnishi, Takashi Ntiamoah, Peter Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| description |
CC-BY |
| format |
Book |
| author |
Ohnishi, Chie Ohnishi, Takashi Ntiamoah, Peter |
| author_facet |
Ohnishi, Chie Ohnishi, Takashi Ntiamoah, Peter |
| author_sort |
Ohnishi, Chie |
| title |
Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| title_short |
Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| title_full |
Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| title_fullStr |
Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| title_full_unstemmed |
Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| title_sort |
standardizing her2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning |
| publisher |
Springer |
| publishDate |
2023 |
| url |
https://link.springer.com/article/10.1186/s42649-023-00091-8 https://dlib.phenikaa-uni.edu.vn/handle/PNK/9064 |
| _version_ |
1779307151590686720 |
| score |
8.891787 |