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Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning

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Bibliographic Details
Main Authors: Ohnishi, Chie, Ohnishi, Takashi, Ntiamoah, Peter
Format: Book
Language:English
Published: Springer 2023
Subjects:
IHC-CS
CCS
Online Access:https://link.springer.com/article/10.1186/s42649-023-00091-8
https://dlib.phenikaa-uni.edu.vn/handle/PNK/9064
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spelling oai:localhost:PNK-90642023-09-18T04:10:57Z Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning Ohnishi, Chie Ohnishi, Takashi Ntiamoah, Peter IHC-CS CCS CC-BY In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for calibrating the color variation due to the scanner. 2023-09-18T04:10:57Z 2023-09-18T04:10:57Z 2023 Book https://link.springer.com/article/10.1186/s42649-023-00091-8 https://dlib.phenikaa-uni.edu.vn/handle/PNK/9064 en application/pdf Springer
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic IHC-CS
CCS
spellingShingle IHC-CS
CCS
Ohnishi, Chie
Ohnishi, Takashi
Ntiamoah, Peter
Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
description CC-BY
format Book
author Ohnishi, Chie
Ohnishi, Takashi
Ntiamoah, Peter
author_facet Ohnishi, Chie
Ohnishi, Takashi
Ntiamoah, Peter
author_sort Ohnishi, Chie
title Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
title_short Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
title_full Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
title_fullStr Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
title_full_unstemmed Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
title_sort standardizing her2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
publisher Springer
publishDate 2023
url https://link.springer.com/article/10.1186/s42649-023-00091-8
https://dlib.phenikaa-uni.edu.vn/handle/PNK/9064
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score 8.8894005

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