DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma
CC BY
Lưu vào:
| Tác giả chính: | , , |
|---|---|
| Định dạng: | Sách |
| Ngôn ngữ: | English |
| Nhà xuất bản: |
Springer
2023
|
| Chủ đề: | |
| Truy cập trực tuyến: | https://link.springer.com/article/10.1186/s40543-023-00383-4 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8227 |
| Từ khóa: |
Thêm từ khóa
Không có từ khóa, Hãy là người đầu tiên đánh dấu biểu ghi này!
|
| id |
oai:localhost:PNK-8227 |
|---|---|
| record_format |
dspace |
| spelling |
oai:localhost:PNK-82272023-04-21T09:27:36Z DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma Yi, Mei Jun, Zhang Li, Zhu RCA DiRCA CC BY Recent studies have reported that miRNA plays an important role in immune response and immune repair after trauma. By regulating the expression of related target genes, miRNA regulates the production, proliferation, development and immune response of immune cells. Therefore, it is in urgent demand to develop an novel method for miRNA analysis. Rolling circle amplification (RCA), as an attractive isothermal signal amplification strategy, has been widely utilized in constructing miRNA detection assays. However, accurate and sensitive miRNA quantitative determination remains a huge challenge for RCA based approaches. Herein, we propose a DSN enzyme based signal cycle initiated Rolling Circle Amplification assay (DiRCA) for sensitive and accurate miRNA detection. In DiRCA, target miRNA unfolds hairpin structure probe in the detection scaffold, forming a RNA–DNA duplex. 2023-04-21T09:27:36Z 2023-04-21T09:27:36Z 2023 Book https://link.springer.com/article/10.1186/s40543-023-00383-4 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8227 en application/pdf Springer |
| institution |
Digital Phenikaa |
| collection |
Digital Phenikaa |
| language |
English |
| topic |
RCA DiRCA |
| spellingShingle |
RCA DiRCA Yi, Mei Jun, Zhang Li, Zhu DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma |
| description |
CC BY |
| format |
Book |
| author |
Yi, Mei Jun, Zhang Li, Zhu |
| author_facet |
Yi, Mei Jun, Zhang Li, Zhu |
| author_sort |
Yi, Mei |
| title |
DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma |
| title_short |
DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma |
| title_full |
DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma |
| title_fullStr |
DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma |
| title_full_unstemmed |
DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma |
| title_sort |
dsn enzyme recognition initiated rolling circle amplification (dirca) for accurate mirna detection in immune response and immune repair after trauma |
| publisher |
Springer |
| publishDate |
2023 |
| url |
https://link.springer.com/article/10.1186/s40543-023-00383-4 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8227 |
| _version_ |
1763815061043806208 |
| score |
8.891145 |