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DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma

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Bibliographic Details
Main Authors: Yi, Mei, Jun, Zhang, Li, Zhu
Format: Book
Language:English
Published: Springer 2023
Subjects:
RCA
DiRCA
Online Access:https://link.springer.com/article/10.1186/s40543-023-00383-4
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8227
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spelling oai:localhost:PNK-82272023-04-21T09:27:36Z DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma Yi, Mei Jun, Zhang Li, Zhu RCA DiRCA CC BY Recent studies have reported that miRNA plays an important role in immune response and immune repair after trauma. By regulating the expression of related target genes, miRNA regulates the production, proliferation, development and immune response of immune cells. Therefore, it is in urgent demand to develop an novel method for miRNA analysis. Rolling circle amplification (RCA), as an attractive isothermal signal amplification strategy, has been widely utilized in constructing miRNA detection assays. However, accurate and sensitive miRNA quantitative determination remains a huge challenge for RCA based approaches. Herein, we propose a DSN enzyme based signal cycle initiated Rolling Circle Amplification assay (DiRCA) for sensitive and accurate miRNA detection. In DiRCA, target miRNA unfolds hairpin structure probe in the detection scaffold, forming a RNA–DNA duplex. 2023-04-21T09:27:36Z 2023-04-21T09:27:36Z 2023 Book https://link.springer.com/article/10.1186/s40543-023-00383-4 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8227 en application/pdf Springer
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic RCA
DiRCA
spellingShingle RCA
DiRCA
Yi, Mei
Jun, Zhang
Li, Zhu
DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma
description CC BY
format Book
author Yi, Mei
Jun, Zhang
Li, Zhu
author_facet Yi, Mei
Jun, Zhang
Li, Zhu
author_sort Yi, Mei
title DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma
title_short DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma
title_full DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma
title_fullStr DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma
title_full_unstemmed DSN enzyme recognition initiated rolling circle amplification (DiRCA) for accurate miRNA detection in immune response and immune repair after trauma
title_sort dsn enzyme recognition initiated rolling circle amplification (dirca) for accurate mirna detection in immune response and immune repair after trauma
publisher Springer
publishDate 2023
url https://link.springer.com/article/10.1186/s40543-023-00383-4
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8227
_version_ 1763815061043806208
score 8.891145

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