Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce
CC BY
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| Định dạng: | Sách |
| Ngôn ngữ: | English |
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Springer
2023
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| Truy cập trực tuyến: | https://link.springer.com/article/10.1186/s13068-023-02316-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8194 |
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oai:localhost:PNK-81942023-04-21T03:25:41Z Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce Fabio, Caputo Monika, Tõlgo Polina, Naidjonoka LPMO9s LPMO-containing Cellic CTec2 cocktails CC BY To realize the full potential of softwood-based forest biorefineries, the bottlenecks of enzymatic saccharification of softwood need to be better understood. Here, we investigated the potential of lytic polysaccharide monooxygenases (LPMO9s) in softwood saccharification. Norway spruce was steam-pretreated at three different severities, leading to varying hemicellulose retention, lignin condensation, and cellulose ultrastructure. Hydrolyzability of the three substrates was assessed after pretreatment and after an additional knife-milling step, comparing the efficiency of cellulolytic Celluclast + Novozym 188 and LPMO-containing Cellic CTec2 cocktails. The role of Thermoascus aurantiacus TaLPMO9 in saccharification was assessed through time-course analysis of sugar release and accumulation of oxidized sugars, as well as wide-angle X-ray scattering analysis of cellulose ultrastructural changes. 2023-04-21T03:25:41Z 2023-04-21T03:25:41Z 2023 Book https://link.springer.com/article/10.1186/s13068-023-02316-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8194 en application/pdf Springer |
| institution |
Digital Phenikaa |
| collection |
Digital Phenikaa |
| language |
English |
| topic |
LPMO9s LPMO-containing Cellic CTec2 cocktails |
| spellingShingle |
LPMO9s LPMO-containing Cellic CTec2 cocktails Fabio, Caputo Monika, Tõlgo Polina, Naidjonoka Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce |
| description |
CC BY |
| format |
Book |
| author |
Fabio, Caputo Monika, Tõlgo Polina, Naidjonoka |
| author_facet |
Fabio, Caputo Monika, Tõlgo Polina, Naidjonoka |
| author_sort |
Fabio, Caputo |
| title |
Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce |
| title_short |
Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce |
| title_full |
Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce |
| title_fullStr |
Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce |
| title_full_unstemmed |
Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce |
| title_sort |
investigating the role of aa9 lpmos in enzymatic hydrolysis of differentially steam-pretreated spruce |
| publisher |
Springer |
| publishDate |
2023 |
| url |
https://link.springer.com/article/10.1186/s13068-023-02316-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8194 |
| _version_ |
1763815058895273984 |
| score |
8.891695 |