Cover Image

Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce

CC BY

Saved in:
Bibliographic Details
Main Authors: Fabio, Caputo, Monika, Tõlgo, Polina, Naidjonoka
Format: Book
Language:English
Published: Springer 2023
Subjects:
LPMO9s
LPMO-containing Cellic CTec2 cocktails
Online Access:https://link.springer.com/article/10.1186/s13068-023-02316-0
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8194
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:localhost:PNK-8194
record_format dspace
spelling oai:localhost:PNK-81942023-04-21T03:25:41Z Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce Fabio, Caputo Monika, Tõlgo Polina, Naidjonoka LPMO9s LPMO-containing Cellic CTec2 cocktails CC BY To realize the full potential of softwood-based forest biorefineries, the bottlenecks of enzymatic saccharification of softwood need to be better understood. Here, we investigated the potential of lytic polysaccharide monooxygenases (LPMO9s) in softwood saccharification. Norway spruce was steam-pretreated at three different severities, leading to varying hemicellulose retention, lignin condensation, and cellulose ultrastructure. Hydrolyzability of the three substrates was assessed after pretreatment and after an additional knife-milling step, comparing the efficiency of cellulolytic Celluclast + Novozym 188 and LPMO-containing Cellic CTec2 cocktails. The role of Thermoascus aurantiacus TaLPMO9 in saccharification was assessed through time-course analysis of sugar release and accumulation of oxidized sugars, as well as wide-angle X-ray scattering analysis of cellulose ultrastructural changes. 2023-04-21T03:25:41Z 2023-04-21T03:25:41Z 2023 Book https://link.springer.com/article/10.1186/s13068-023-02316-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8194 en application/pdf Springer
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic LPMO9s
LPMO-containing Cellic CTec2 cocktails
spellingShingle LPMO9s
LPMO-containing Cellic CTec2 cocktails
Fabio, Caputo
Monika, Tõlgo
Polina, Naidjonoka
Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce
description CC BY
format Book
author Fabio, Caputo
Monika, Tõlgo
Polina, Naidjonoka
author_facet Fabio, Caputo
Monika, Tõlgo
Polina, Naidjonoka
author_sort Fabio, Caputo
title Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce
title_short Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce
title_full Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce
title_fullStr Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce
title_full_unstemmed Investigating the role of AA9 LPMOs in enzymatic hydrolysis of differentially steam-pretreated spruce
title_sort investigating the role of aa9 lpmos in enzymatic hydrolysis of differentially steam-pretreated spruce
publisher Springer
publishDate 2023
url https://link.springer.com/article/10.1186/s13068-023-02316-0
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8194
_version_ 1763815058895273984
score 8.881002

Similar Items