Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail
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| Định dạng: | Sách |
| Ngôn ngữ: | English |
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Springer
2023
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| Truy cập trực tuyến: | https://link.springer.com/article/10.1007/s11696-022-02658-3 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8164 |
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oai:localhost:PNK-81642023-04-20T08:42:47Z Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail Paola Di, Matteo Francesca, Luziatelli Martina, Bortolami DSC crude cellulase cocktail CC BY Differential scanning calorimetry (DSC) was used as an efficient and rapid tool in studying the conformational transitions between the folded and unfolded structures of cellulolytic enzymes. The thermal properties of two crude hydrolytic enzyme cocktails containing extracellular cellulases from Trichoderma longibrachiatum DIBAF-10 were analyzed and compared with three commercial cellulase preparations. Differences in the thermal behavior of fungal cellulases in the liquid phase, freeze-dried state, liquid formulations in sodium citrate buffer (pH 4.8), and contact with cellulose, carboxymethyl cellulose, and cellobiose were evaluated. DSC profiles of cellulases from the DIBAF-10 strain provided important thermodynamic information about the thermal stability of the included proteins. Crude enzyme cocktails underwent a reproducible and irreversible exothermic aggregation phenomenon at 52.45 ± 0.90 °C like commercial β-glucosidase. 2023-04-20T08:42:47Z 2023-04-20T08:42:47Z 2023 Book https://link.springer.com/article/10.1007/s11696-022-02658-3 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8164 en application/pdf Springer |
| institution |
Digital Phenikaa |
| collection |
Digital Phenikaa |
| language |
English |
| topic |
DSC crude cellulase cocktail |
| spellingShingle |
DSC crude cellulase cocktail Paola Di, Matteo Francesca, Luziatelli Martina, Bortolami Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail |
| description |
CC BY |
| format |
Book |
| author |
Paola Di, Matteo Francesca, Luziatelli Martina, Bortolami |
| author_facet |
Paola Di, Matteo Francesca, Luziatelli Martina, Bortolami |
| author_sort |
Paola Di, Matteo |
| title |
Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail |
| title_short |
Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail |
| title_full |
Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail |
| title_fullStr |
Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail |
| title_full_unstemmed |
Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail |
| title_sort |
differential scanning calorimetry (dsc) as a tool for studying thermal properties of a crude cellulase cocktail |
| publisher |
Springer |
| publishDate |
2023 |
| url |
https://link.springer.com/article/10.1007/s11696-022-02658-3 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8164 |
| _version_ |
1763724455655243776 |
| score |
8.891145 |