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Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail

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Bibliographic Details
Main Authors: Paola Di, Matteo, Francesca, Luziatelli, Martina, Bortolami
Format: Book
Language:English
Published: Springer 2023
Subjects:
DSC
crude cellulase cocktail
Online Access:https://link.springer.com/article/10.1007/s11696-022-02658-3
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8164
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spelling oai:localhost:PNK-81642023-04-20T08:42:47Z Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail Paola Di, Matteo Francesca, Luziatelli Martina, Bortolami DSC crude cellulase cocktail CC BY Differential scanning calorimetry (DSC) was used as an efficient and rapid tool in studying the conformational transitions between the folded and unfolded structures of cellulolytic enzymes. The thermal properties of two crude hydrolytic enzyme cocktails containing extracellular cellulases from Trichoderma longibrachiatum DIBAF-10 were analyzed and compared with three commercial cellulase preparations. Differences in the thermal behavior of fungal cellulases in the liquid phase, freeze-dried state, liquid formulations in sodium citrate buffer (pH 4.8), and contact with cellulose, carboxymethyl cellulose, and cellobiose were evaluated. DSC profiles of cellulases from the DIBAF-10 strain provided important thermodynamic information about the thermal stability of the included proteins. Crude enzyme cocktails underwent a reproducible and irreversible exothermic aggregation phenomenon at 52.45 ± 0.90 °C like commercial β-glucosidase. 2023-04-20T08:42:47Z 2023-04-20T08:42:47Z 2023 Book https://link.springer.com/article/10.1007/s11696-022-02658-3 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8164 en application/pdf Springer
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic DSC
crude cellulase cocktail
spellingShingle DSC
crude cellulase cocktail
Paola Di, Matteo
Francesca, Luziatelli
Martina, Bortolami
Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail
description CC BY
format Book
author Paola Di, Matteo
Francesca, Luziatelli
Martina, Bortolami
author_facet Paola Di, Matteo
Francesca, Luziatelli
Martina, Bortolami
author_sort Paola Di, Matteo
title Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail
title_short Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail
title_full Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail
title_fullStr Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail
title_full_unstemmed Differential scanning calorimetry (DSC) as a tool for studying thermal properties of a crude cellulase cocktail
title_sort differential scanning calorimetry (dsc) as a tool for studying thermal properties of a crude cellulase cocktail
publisher Springer
publishDate 2023
url https://link.springer.com/article/10.1007/s11696-022-02658-3
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8164
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score 8.8894005

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