Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system
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| Ngôn ngữ: | English |
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Springer
2023
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| Truy cập trực tuyến: | https://link.springer.com/article/10.1007/s00216-023-04521-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8154 |
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oai:localhost:PNK-81542023-04-20T07:39:14Z Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system Vanesa, Román-Pizarro Alba María, Carrión-Escudero Ángela, Écija-Arenas GPx MREµR CC BY A stopped-flow microfluidic system to monitor glutathione peroxidase (GPx) activity and evaluate potential inhibitors of the enzyme has been developed based on the integration of the microfluidic chip in the reaction/detection zone. This integration supposes the physical alignment at the optimal location of the microfluidic channel, both the magnetically retained enzyme microreactor (MREµR) and the remote luminescence detection using a focused bifurcated fiber optic bundle (BFOB) connected to a conventional spectrofluorometer detector. The method is based on the coupling of two competitive oxidative chemical reactions, in which glutathione (GSH) and homovanillic acid (HVA) competed for their interaction with hydrogen peroxide in the presence of the magnetically retained GPx-MNPs. 2023-04-20T07:39:14Z 2023-04-20T07:39:14Z 2023 Book https://link.springer.com/article/10.1007/s00216-023-04521-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8154 en application/pdf Springer |
| institution |
Digital Phenikaa |
| collection |
Digital Phenikaa |
| language |
English |
| topic |
GPx MREµR |
| spellingShingle |
GPx MREµR Vanesa, Román-Pizarro Alba María, Carrión-Escudero Ángela, Écija-Arenas Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system |
| description |
CC BY |
| format |
Book |
| author |
Vanesa, Román-Pizarro Alba María, Carrión-Escudero Ángela, Écija-Arenas |
| author_facet |
Vanesa, Román-Pizarro Alba María, Carrión-Escudero Ángela, Écija-Arenas |
| author_sort |
Vanesa, Román-Pizarro |
| title |
Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system |
| title_short |
Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system |
| title_full |
Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system |
| title_fullStr |
Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system |
| title_full_unstemmed |
Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system |
| title_sort |
study of the inhibition effects on glutathione peroxidase immobilized on mnps using a stopped-flow microfluidic system |
| publisher |
Springer |
| publishDate |
2023 |
| url |
https://link.springer.com/article/10.1007/s00216-023-04521-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8154 |
| _version_ |
1763724455005126656 |
| score |
8.887836 |