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Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system

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Bibliographic Details
Main Authors: Vanesa, Román-Pizarro, Alba María, Carrión-Escudero, Ángela, Écija-Arenas
Format: Book
Language:English
Published: Springer 2023
Subjects:
GPx
MREµR
Online Access:https://link.springer.com/article/10.1007/s00216-023-04521-0
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8154
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spelling oai:localhost:PNK-81542023-04-20T07:39:14Z Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system Vanesa, Román-Pizarro Alba María, Carrión-Escudero Ángela, Écija-Arenas GPx MREµR CC BY A stopped-flow microfluidic system to monitor glutathione peroxidase (GPx) activity and evaluate potential inhibitors of the enzyme has been developed based on the integration of the microfluidic chip in the reaction/detection zone. This integration supposes the physical alignment at the optimal location of the microfluidic channel, both the magnetically retained enzyme microreactor (MREµR) and the remote luminescence detection using a focused bifurcated fiber optic bundle (BFOB) connected to a conventional spectrofluorometer detector. The method is based on the coupling of two competitive oxidative chemical reactions, in which glutathione (GSH) and homovanillic acid (HVA) competed for their interaction with hydrogen peroxide in the presence of the magnetically retained GPx-MNPs. 2023-04-20T07:39:14Z 2023-04-20T07:39:14Z 2023 Book https://link.springer.com/article/10.1007/s00216-023-04521-0 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8154 en application/pdf Springer
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic GPx
MREµR
spellingShingle GPx
MREµR
Vanesa, Román-Pizarro
Alba María, Carrión-Escudero
Ángela, Écija-Arenas
Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system
description CC BY
format Book
author Vanesa, Román-Pizarro
Alba María, Carrión-Escudero
Ángela, Écija-Arenas
author_facet Vanesa, Román-Pizarro
Alba María, Carrión-Escudero
Ángela, Écija-Arenas
author_sort Vanesa, Román-Pizarro
title Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system
title_short Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system
title_full Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system
title_fullStr Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system
title_full_unstemmed Study of the inhibition effects on glutathione peroxidase immobilized on MNPs using a stopped-flow microfluidic system
title_sort study of the inhibition effects on glutathione peroxidase immobilized on mnps using a stopped-flow microfluidic system
publisher Springer
publishDate 2023
url https://link.springer.com/article/10.1007/s00216-023-04521-0
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8154
_version_ 1763724455005126656
score 8.891145

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