Cover Image

Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement

Intramuscular delivery of human adenovirus (HAdV)-based vaccines leads to rapid recruitment of neutrophils, which then release antimicrobial peptides/proteins (AMPs). How these AMPs influence vaccine efficacy over the subsequent 24 h is poorly understood. In this study, we asked if human neutrophil...

Full description

Saved in:
Bibliographic Details
Main Authors: Karsten, Eichholz, Tuan, Hiep Tran, Coraline ,Chéneau, Tran, Thi Thu Phuong, Océane, Paris, Martine, Pugniere, Eric, J. Kremer
Format: Bài trích
Language:English
Published: American Society for Microbiology 2022
Subjects:
Adenovirus-α-Defensin
NLRP3-Associated
Online Access:https://journals.asm.org/doi/10.1128/jvi.01850-21
https://dlib.phenikaa-uni.edu.vn/handle/PNK/5735
https://doi.org/10.1128/jvi.01850-21
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:localhost:PNK-5735
record_format dspace
spelling oai:localhost:PNK-57352022-08-17T05:54:52Z Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement Karsten, Eichholz Tuan, Hiep Tran Coraline ,Chéneau Tran, Thi Thu Phuong Océane, Paris Martine, Pugniere Eric, J. Kremer Adenovirus-α-Defensin NLRP3-Associated Intramuscular delivery of human adenovirus (HAdV)-based vaccines leads to rapid recruitment of neutrophils, which then release antimicrobial peptides/proteins (AMPs). How these AMPs influence vaccine efficacy over the subsequent 24 h is poorly understood. In this study, we asked if human neutrophil protein 1 (HNP-1), an α-defensin that influences direct and indirect innate immune responses to a range of pathogens, impacts the response of human phagocytes to three HAdV species/types (HAdV-C5, -D26, -B35). We show that HNP-1 binds to the capsids and redirects HAdV-C5, -D26, and -B35 to Toll-like receptor 4 (TLR4), which leads to internalization, an NLRP3-mediated inflammasome response, and interleukin 1 beta (IL-1β) release. Surprisingly, IL-1β release was not associated with notable disruption of plasma membrane integrity. These data further our understanding of HAdV vaccine immunogenicity and may provide pathways to extend the efficacy 2022-05-05T07:26:14Z 2022-05-05T07:26:14Z 2022 Bài trích https://journals.asm.org/doi/10.1128/jvi.01850-21 https://dlib.phenikaa-uni.edu.vn/handle/PNK/5735 https://doi.org/10.1128/jvi.01850-21 en American Society for Microbiology
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic Adenovirus-α-Defensin
NLRP3-Associated
spellingShingle Adenovirus-α-Defensin
NLRP3-Associated
Karsten, Eichholz
Tuan, Hiep Tran
Coraline ,Chéneau
Tran, Thi Thu Phuong
Océane, Paris
Martine, Pugniere
Eric, J. Kremer
Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement
description Intramuscular delivery of human adenovirus (HAdV)-based vaccines leads to rapid recruitment of neutrophils, which then release antimicrobial peptides/proteins (AMPs). How these AMPs influence vaccine efficacy over the subsequent 24 h is poorly understood. In this study, we asked if human neutrophil protein 1 (HNP-1), an α-defensin that influences direct and indirect innate immune responses to a range of pathogens, impacts the response of human phagocytes to three HAdV species/types (HAdV-C5, -D26, -B35). We show that HNP-1 binds to the capsids and redirects HAdV-C5, -D26, and -B35 to Toll-like receptor 4 (TLR4), which leads to internalization, an NLRP3-mediated inflammasome response, and interleukin 1 beta (IL-1β) release. Surprisingly, IL-1β release was not associated with notable disruption of plasma membrane integrity. These data further our understanding of HAdV vaccine immunogenicity and may provide pathways to extend the efficacy
format Bài trích
author Karsten, Eichholz
Tuan, Hiep Tran
Coraline ,Chéneau
Tran, Thi Thu Phuong
Océane, Paris
Martine, Pugniere
Eric, J. Kremer
author_facet Karsten, Eichholz
Tuan, Hiep Tran
Coraline ,Chéneau
Tran, Thi Thu Phuong
Océane, Paris
Martine, Pugniere
Eric, J. Kremer
author_sort Karsten, Eichholz
title Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement
title_short Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement
title_full Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement
title_fullStr Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement
title_full_unstemmed Adenovirus-α-Defensin Complexes Induce NLRP3-Associated Maturation of Human Phagocytes via Toll-Like Receptor 4 Engagement
title_sort adenovirus-α-defensin complexes induce nlrp3-associated maturation of human phagocytes via toll-like receptor 4 engagement
publisher American Society for Microbiology
publishDate 2022
url https://journals.asm.org/doi/10.1128/jvi.01850-21
https://dlib.phenikaa-uni.edu.vn/handle/PNK/5735
https://doi.org/10.1128/jvi.01850-21
_version_ 1751856313681313792
score 8.891145

Similar Items