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Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls

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Main Authors: Sandrine, Reymond, Lyssia, Gruaz, Jean-Charles, Sanchez
Format: Book
Language:English
Published: Springer 2023
Subjects:
EVs
LC–MS/MS
Online Access:https://link.springer.com/article/10.1007/s00216-023-04684-w
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8216
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spelling oai:localhost:PNK-82162023-04-21T08:00:08Z Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls Sandrine, Reymond Lyssia, Gruaz Jean-Charles, Sanchez EVs LC–MS/MS CC BY Blood extracellular vesicles (EVs) play essential roles in cell–cell communication and their molecular cargo is a promising source of disease biomarkers. However, proteomic characterization of plasma-derived EVs is challenged by the presence of highly abundant plasma proteins, which limits the detection of less abundant proteins, and by the low number of EVs in biological fluids. The aim of this study was to investigate if the removal of abundant plasma proteins prior to EV isolation could improve plasma-derived EV characterization by LC–MS/MS and expand the proteome coverage. Plasma depletion was performed using a single-use spin column and EVs were isolated from only 100 µL of non-depleted and depleted plasma by size exclusion chromatography. 2023-04-21T08:00:08Z 2023-04-21T08:00:08Z 2023 Book https://link.springer.com/article/10.1007/s00216-023-04684-w https://dlib.phenikaa-uni.edu.vn/handle/PNK/8216 en application/pdf Springer
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic EVs
LC–MS/MS
spellingShingle EVs
LC–MS/MS
Sandrine, Reymond
Lyssia, Gruaz
Jean-Charles, Sanchez
Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
description CC BY
format Book
author Sandrine, Reymond
Lyssia, Gruaz
Jean-Charles, Sanchez
author_facet Sandrine, Reymond
Lyssia, Gruaz
Jean-Charles, Sanchez
author_sort Sandrine, Reymond
title Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
title_short Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
title_full Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
title_fullStr Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
title_full_unstemmed Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
title_sort depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls
publisher Springer
publishDate 2023
url https://link.springer.com/article/10.1007/s00216-023-04684-w
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8216
_version_ 1763815060276248576
score 8.889492

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