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First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin

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Main Authors: Cristina Muñoz-San, Martín, Ana, Montero-Calle, María, Garranzo-Asensio
Format: Book
Language:English
Published: Springer 2023
Subjects:
HRP
LODs
Online Access:https://link.springer.com/article/10.1007/s00216-022-04397-6
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8126
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spelling oai:localhost:PNK-81262023-04-20T03:31:04Z First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin Cristina Muñoz-San, Martín Ana, Montero-Calle María, Garranzo-Asensio HRP LODs CC BY The glycosylation status of proteins is increasingly used as biomarker to improve the reliability in the diagnosis and prognosis of diseases as relevant as cancer. This feeds the need for tools that allow its simple and reliable analysis and are compatible with applicability in the clinic. With this objective in mind, this work reports the first bioelectronic immunoplatforms described to date for the determination of glycosylated haptoglobin (Hp) and the simultaneous determination of total and glycosylated Hp. The bioelectronic immunoplatform is based on the implementation of non-competitive bioassays using two different antibodies or an antibody and a lectin on the surface of commercial magnetic microcarriers. The resulting bioconjugates are labeled with the horseradish peroxidase (HRP) enzyme, and after their magnetic capture on disposable electroplatforms, the amperometric transduction using the H2O2/hydroquinone (HQ) system allows the single or multiple detection. The developed immunoplatform achieves limits of detection (LODs) of 0.07 and 0.46 ng mL−1 for total and glycosylated Hp in buffer solution, respectively. 2023-04-20T03:31:04Z 2023-04-20T03:31:04Z 2023 Book https://link.springer.com/article/10.1007/s00216-022-04397-6 https://dlib.phenikaa-uni.edu.vn/handle/PNK/8126 en application/pdf Springer
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic HRP
LODs
spellingShingle HRP
LODs
Cristina Muñoz-San, Martín
Ana, Montero-Calle
María, Garranzo-Asensio
First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
description CC BY
format Book
author Cristina Muñoz-San, Martín
Ana, Montero-Calle
María, Garranzo-Asensio
author_facet Cristina Muñoz-San, Martín
Ana, Montero-Calle
María, Garranzo-Asensio
author_sort Cristina Muñoz-San, Martín
title First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
title_short First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
title_full First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
title_fullStr First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
title_full_unstemmed First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
title_sort first bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
publisher Springer
publishDate 2023
url https://link.springer.com/article/10.1007/s00216-022-04397-6
https://dlib.phenikaa-uni.edu.vn/handle/PNK/8126
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score 8.891145

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