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CRISPR-Cas system in microbial hosts for terpenoid production

Terpenoids represent the largest group of secondary metabolites with variable structures and functions. Terpenoids are well known for their beneficial application in human life, such as pharmaceutical products, vitamins, hormones, anticancer drugs, cosmetics, flavors and fragrances, foods, agricultu...

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Bibliographic Details
Main Author: Luan Luong, Chu
Format: Bài trích
Language:English
Published: Informa UK Limited 2022
Subjects:
Terpenoids
Escherichia coli
Online Access:https://www.tandfonline.com/doi/abs/10.1080/07388551.2021.1995318?journalCode=ibty20
https://dlib.phenikaa-uni.edu.vn/handle/PNK/5870
https://doi.org/10.1080/07388551.2021.1995318
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spelling oai:localhost:PNK-58702022-08-17T05:54:54Z CRISPR-Cas system in microbial hosts for terpenoid production Luan Luong, Chu Terpenoids Escherichia coli Terpenoids represent the largest group of secondary metabolites with variable structures and functions. Terpenoids are well known for their beneficial application in human life, such as pharmaceutical products, vitamins, hormones, anticancer drugs, cosmetics, flavors and fragrances, foods, agriculture, and biofuels. Recently, engineering microbial cells have been provided with a sustainable approach to produce terpenoids with high yields. Noticeably, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has emerged as one of the most efficient genome-editing technologies to engineer microorganisms for improving terpenoid production. In this review, we summarize the application of the CRISPR-Cas system for the production of terpenoids in microbial hosts such as Escherichia coli, Saccharomyces cerevisiae, Corynebacterium glutamicum, and Pseudomonas putida. CRISPR-Cas9 deactivated Cas9 (dCas9)-based CRISPR (CRISPRi), and the dCas9-based activator (CRISPRa) have been used in either individual or combinatorial systems to control the metabolic flux for enhancing the production of terpenoids. Finally, the prospects of using the CRISPR-Cas system in terpenoid production are also discussed. 2022-07-13T01:59:45Z 2022-07-13T01:59:45Z 2022 Bài trích https://www.tandfonline.com/doi/abs/10.1080/07388551.2021.1995318?journalCode=ibty20 https://dlib.phenikaa-uni.edu.vn/handle/PNK/5870 https://doi.org/10.1080/07388551.2021.1995318 en Informa UK Limited
institution Digital Phenikaa
collection Digital Phenikaa
language English
topic Terpenoids
Escherichia coli
spellingShingle Terpenoids
Escherichia coli
Luan Luong, Chu
CRISPR-Cas system in microbial hosts for terpenoid production
description Terpenoids represent the largest group of secondary metabolites with variable structures and functions. Terpenoids are well known for their beneficial application in human life, such as pharmaceutical products, vitamins, hormones, anticancer drugs, cosmetics, flavors and fragrances, foods, agriculture, and biofuels. Recently, engineering microbial cells have been provided with a sustainable approach to produce terpenoids with high yields. Noticeably, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has emerged as one of the most efficient genome-editing technologies to engineer microorganisms for improving terpenoid production. In this review, we summarize the application of the CRISPR-Cas system for the production of terpenoids in microbial hosts such as Escherichia coli, Saccharomyces cerevisiae, Corynebacterium glutamicum, and Pseudomonas putida. CRISPR-Cas9 deactivated Cas9 (dCas9)-based CRISPR (CRISPRi), and the dCas9-based activator (CRISPRa) have been used in either individual or combinatorial systems to control the metabolic flux for enhancing the production of terpenoids. Finally, the prospects of using the CRISPR-Cas system in terpenoid production are also discussed.
format Bài trích
author Luan Luong, Chu
author_facet Luan Luong, Chu
author_sort Luan Luong, Chu
title CRISPR-Cas system in microbial hosts for terpenoid production
title_short CRISPR-Cas system in microbial hosts for terpenoid production
title_full CRISPR-Cas system in microbial hosts for terpenoid production
title_fullStr CRISPR-Cas system in microbial hosts for terpenoid production
title_full_unstemmed CRISPR-Cas system in microbial hosts for terpenoid production
title_sort crispr-cas system in microbial hosts for terpenoid production
publisher Informa UK Limited
publishDate 2022
url https://www.tandfonline.com/doi/abs/10.1080/07388551.2021.1995318?journalCode=ibty20
https://dlib.phenikaa-uni.edu.vn/handle/PNK/5870
https://doi.org/10.1080/07388551.2021.1995318
_version_ 1751856285311041536
score 8.881002

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