Staff View: Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent

Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent

Magnoliae Flos is a traditional herbal medicine used to treat nasal congestion associated with headache, empyema, and allergic rhinitis. In our preliminary screening of crude drugs used in Japanese Kampo formulas for melanin synthesis, the methanol extract of Magnoliae Flos was found to exhibit stro...

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Main Authors:	Takuhiro, Uto, Nguyen, Huu Tung, Tomoe, Ohta, Yukihiro, Shoyama
Format:	Bài trích
Language:	English
Published:	Georg Thieme Verlag KG 2022
Subjects:	Magnolia biondii Magnoliaceae
Online Access:	https://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-1740-7325 https://dlib.phenikaa-uni.edu.vn/handle/PNK/5761 https://doi.org/10.1055/a-1740-7325
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spelling	oai:localhost:PNK-57612022-08-17T05:54:53Z Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent Takuhiro, Uto Nguyen, Huu Tung Tomoe, Ohta Yukihiro, Shoyama Magnolia biondii Magnoliaceae Magnoliae Flos is a traditional herbal medicine used to treat nasal congestion associated with headache, empyema, and allergic rhinitis. In our preliminary screening of crude drugs used in Japanese Kampo formulas for melanin synthesis, the methanol extract of Magnoliae Flos was found to exhibit strong melanin synthesis activity. However, there have been no studies evaluating the effects of Magnoliae Flos or its constituents on melanogenesis. The present study aimed to isolate the active compounds from Magnoliae Flos that activate melanin synthesis in melanoma cells and three-dimensional human skin equivalent, and to investigate the molecular mechanism underlying melanin induction. The methanol extract of Magnoliae Flos induced an increase of melanin content in both B16-F1 and HMV-II cells. A comparison of melanin induction by three fractions prepared from the extract showed that the ethyl acetate fraction markedly induced melanin synthesis. Bioassay-guided separation of the ethyl acetate fraction resulted in the isolation of seven lignans (1 – 7). Among them, (+)-magnolin (5) strongly induced melanin synthesis and intracellular tyrosinase activity. Furthermore, the ethyl acetate fraction and 5 clearly induced melanin content in a three-dimensional human skin equivalent. Molecular analysis revealed that 5 triggered the protein expression of tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. Further analysis of transcriptional factors and signaling pathways demonstrated that 5 induces the protein expression of tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2 activated by the protein kinase A- and p38 mitogen-activated protein kinase-dependent pathways, leading to cAMP-responsive element-binding protein phosphorylation and microphthalmia-associated transcription factor expression. These findings demonstrate the potential of 5 as a potent therapeutic agent for hypopigmentation. 2022-05-05T07:26:20Z 2022-05-05T07:26:20Z 2022 Bài trích https://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-1740-7325 https://dlib.phenikaa-uni.edu.vn/handle/PNK/5761 https://doi.org/10.1055/a-1740-7325 en Georg Thieme Verlag KG
institution	Digital Phenikaa
collection	Digital Phenikaa
language	English
topic	Magnolia biondii Magnoliaceae
spellingShingle	Magnolia biondii Magnoliaceae Takuhiro, Uto Nguyen, Huu Tung Tomoe, Ohta Yukihiro, Shoyama Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent
description	Magnoliae Flos is a traditional herbal medicine used to treat nasal congestion associated with headache, empyema, and allergic rhinitis. In our preliminary screening of crude drugs used in Japanese Kampo formulas for melanin synthesis, the methanol extract of Magnoliae Flos was found to exhibit strong melanin synthesis activity. However, there have been no studies evaluating the effects of Magnoliae Flos or its constituents on melanogenesis. The present study aimed to isolate the active compounds from Magnoliae Flos that activate melanin synthesis in melanoma cells and three-dimensional human skin equivalent, and to investigate the molecular mechanism underlying melanin induction. The methanol extract of Magnoliae Flos induced an increase of melanin content in both B16-F1 and HMV-II cells. A comparison of melanin induction by three fractions prepared from the extract showed that the ethyl acetate fraction markedly induced melanin synthesis. Bioassay-guided separation of the ethyl acetate fraction resulted in the isolation of seven lignans (1 – 7). Among them, (+)-magnolin (5) strongly induced melanin synthesis and intracellular tyrosinase activity. Furthermore, the ethyl acetate fraction and 5 clearly induced melanin content in a three-dimensional human skin equivalent. Molecular analysis revealed that 5 triggered the protein expression of tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. Further analysis of transcriptional factors and signaling pathways demonstrated that 5 induces the protein expression of tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2 activated by the protein kinase A- and p38 mitogen-activated protein kinase-dependent pathways, leading to cAMP-responsive element-binding protein phosphorylation and microphthalmia-associated transcription factor expression. These findings demonstrate the potential of 5 as a potent therapeutic agent for hypopigmentation.
format	Bài trích
author	Takuhiro, Uto Nguyen, Huu Tung Tomoe, Ohta Yukihiro, Shoyama
author_facet	Takuhiro, Uto Nguyen, Huu Tung Tomoe, Ohta Yukihiro, Shoyama
author_sort	Takuhiro, Uto
title	Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent
title_short	Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent
title_full	Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent
title_fullStr	Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent
title_full_unstemmed	Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent
title_sort	magnolin enhances melanogenesis in melanoma cells and three-dimensional human skin equivalent
publisher	Georg Thieme Verlag KG
publishDate	2022
url	https://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-1740-7325 https://dlib.phenikaa-uni.edu.vn/handle/PNK/5761 https://doi.org/10.1055/a-1740-7325
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score	8.891145

Magnolin Enhances Melanogenesis in Melanoma Cells and Three-Dimensional Human Skin Equivalent

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