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Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection

The study is aimed at investigating the stability of electrochemical and biosensing properties of ZnO nanorod-based platinum screen-printed electrodes (SPEs) applied for detection of bacterial pathogens. The platinum SPEs were designed and patterned according to standard photolithography and lift-of...

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Main Author: Dang Thi Thanh Le
Other Authors: Nguyen Thi Hong Phuc
Published: 2019
Online Access:https://dlib.phenikaa-uni.edu.vn/handle/PNK/313
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spelling oai:localhost:PNK-3132022-08-17T05:54:44Z Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection Dang Thi Thanh Le Nguyen Thi Hong Phuc Matteo Tonezzer Vu Quang Khue Tran Quang Huy Nguyen Duc Hoa  Nguyen Van Hieu The study is aimed at investigating the stability of electrochemical and biosensing properties of ZnO nanorod-based platinum screen-printed electrodes (SPEs) applied for detection of bacterial pathogens. The platinum SPEs were designed and patterned according to standard photolithography and lift-off process on a silicon wafer. ZnO nanorods (NRs) were grown on the platinum working electrode by the hydrothermal method, whereas Salmonella polyclonal antibodies were selected and immobilized onto ZnO NR surface via a crosslinking process. Morphological and structural characteristics of ZnO NRs were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The results showed that the ZnO NRs were grown vertically on platinum electrodes with a diameter around 20-200 nm and a length of 5-7 μm. These modified electrodes were applied for detection of Salmonella enteritidis at a concentration of 103 cfu/mL by electrochemical measurements including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO NR-modified platinum electrodes could detect Salmonella bacteria well with stable measurements, and the signal to noise ratio was much higher than that of 3 : 1. This study indicated that ZnO NR-modified platinum SPEs could be potential for the development of biochips for electrochemical detection of bacterial pathogens. 2019 2020-04-29T07:53:07Z 2020-04-29T07:53:07Z bccb00040 https://dlib.phenikaa-uni.edu.vn/handle/PNK/313 application/pdf
institution Digital Phenikaa
collection Digital Phenikaa
description The study is aimed at investigating the stability of electrochemical and biosensing properties of ZnO nanorod-based platinum screen-printed electrodes (SPEs) applied for detection of bacterial pathogens. The platinum SPEs were designed and patterned according to standard photolithography and lift-off process on a silicon wafer. ZnO nanorods (NRs) were grown on the platinum working electrode by the hydrothermal method, whereas Salmonella polyclonal antibodies were selected and immobilized onto ZnO NR surface via a crosslinking process. Morphological and structural characteristics of ZnO NRs were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The results showed that the ZnO NRs were grown vertically on platinum electrodes with a diameter around 20-200 nm and a length of 5-7 μm. These modified electrodes were applied for detection of Salmonella enteritidis at a concentration of 103 cfu/mL by electrochemical measurements including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO NR-modified platinum electrodes could detect Salmonella bacteria well with stable measurements, and the signal to noise ratio was much higher than that of 3 : 1. This study indicated that ZnO NR-modified platinum SPEs could be potential for the development of biochips for electrochemical detection of bacterial pathogens.
author2 Nguyen Thi Hong Phuc
author_facet Nguyen Thi Hong Phuc
Dang Thi Thanh Le
author Dang Thi Thanh Le
spellingShingle Dang Thi Thanh Le
Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection
author_sort Dang Thi Thanh Le
title Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection
title_short Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection
title_full Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection
title_fullStr Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection
title_full_unstemmed Stable electrochemical measurements of platinum screen-printed electrodes modified with vertical ZnO nanorods for bacterial detection
title_sort stable electrochemical measurements of platinum screen-printed electrodes modified with vertical zno nanorods for bacterial detection
publishDate 2019
url https://dlib.phenikaa-uni.edu.vn/handle/PNK/313
_version_ 1751856268288458752
score 8.891145

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