Hiển thị ở chế độ nhân viên: Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum

Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum

Fagopyrum esculentum (buckwheat) is an annual herb that is commonly cultivated in Vietnam for ornamental and food purposes. Previous studies also indicated the medicinal potential of the plant due to its high content of bioactive compounds. However, every year, more than 300 ha of buckwheat cultivat...

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Tác giả chính:	Cong Pham-Van, Thi-Ngoc-Oanh Le, Thi-Ngoc-Bich Tran, Khanh-Linh Nguyen, Nhien Le-Thi, Tung Nguyen-Huu, Hieu Nguyen-Ngoc
Định dạng:	Bài trích
Ngôn ngữ:	eng
Nhà xuất bản:	Tropical Journal of Natural Product Research 2021
Chủ đề:	Fagopyrum esculentum Chemical constituents HPLC quantification
Truy cập trực tuyến:	https://www.tjnpr.org/viewarticle.aspx?articleid=1721 https://dlib.phenikaa-uni.edu.vn/handle/PNK/2840 http://www.doi.org/10.26538/tjnpr/v5i7.17
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spelling	oai:localhost:PNK-28402022-08-17T05:54:47Z Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum Cong Pham-Van Thi-Ngoc-Oanh Le Thi-Ngoc-Bich Tran Khanh-Linh Nguyen Nhien Le-Thi Tung Nguyen-Huu Hieu Nguyen-Ngoc Fagopyrum esculentum Chemical constituents HPLC quantification Fagopyrum esculentum (buckwheat) is an annual herb that is commonly cultivated in Vietnam for ornamental and food purposes. Previous studies also indicated the medicinal potential of the plant due to its high content of bioactive compounds. However, every year, more than 300 ha of buckwheat cultivation has been discarded after flower festival in North Vietnam. The present study was conducted to clarify the main chemical constituents of F. esculentum aerial parts collected in Ha Giang, Vietnam. The investigation has led to the isolation and identification of nine compounds, specifically flavonoids (rutin, quercetin, quercetin 3-O-rhamnopyranoside, quercetin 3-O-galactopyranoside), lignan (isolariciresinol), phenylpropanoids linking with sucrose (lapathoside A, lapathoside D) and phytosterols (?-sitosterol, and daucosterol). An HPLC quantification method of the major component of F. esculentum leaves, rutin, was also developed. The optimized condition was performed on a Shimadzu LC-20D HPLC system; Shim-pack GIST C18 column (4.6 mm × 250 mm × 5 µm); eluting solvent system (0-20 min:16-26% acetonitrile in water containing 0.1% formic acid); injection volume 20 ?L; flow rate 1.2 mL/ min; column temperature 35°C; UV wavelength 254 nm. The method was also validated by various parameters, including linearity (R2= 0.9995), precision (6 samples; RSD= 1.58%), accuracy (recover rates from 94.4 to 103.9%), limit of detection (LOD= 0.049 ?g/mL), limit of quantification (LOQ= 0.147 ?g/mL). The analytical results showed that the content of rutin in leaf and flower samples were 1.794 ± 0.002% and 1.137 ± 0.002% , respectively. 2021-09-14T07:14:52Z 2021-09-14T07:14:52Z 2021 Bài trích https://www.tjnpr.org/viewarticle.aspx?articleid=1721 https://dlib.phenikaa-uni.edu.vn/handle/PNK/2840 http://www.doi.org/10.26538/tjnpr/v5i7.17 eng application/pdf Tropical Journal of Natural Product Research
institution	Digital Phenikaa
collection	Digital Phenikaa
language	eng
topic	Fagopyrum esculentum Chemical constituents HPLC quantification
spellingShingle	Fagopyrum esculentum Chemical constituents HPLC quantification Cong Pham-Van Thi-Ngoc-Oanh Le Thi-Ngoc-Bich Tran Khanh-Linh Nguyen Nhien Le-Thi Tung Nguyen-Huu Hieu Nguyen-Ngoc Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum
description	Fagopyrum esculentum (buckwheat) is an annual herb that is commonly cultivated in Vietnam for ornamental and food purposes. Previous studies also indicated the medicinal potential of the plant due to its high content of bioactive compounds. However, every year, more than 300 ha of buckwheat cultivation has been discarded after flower festival in North Vietnam. The present study was conducted to clarify the main chemical constituents of F. esculentum aerial parts collected in Ha Giang, Vietnam. The investigation has led to the isolation and identification of nine compounds, specifically flavonoids (rutin, quercetin, quercetin 3-O-rhamnopyranoside, quercetin 3-O-galactopyranoside), lignan (isolariciresinol), phenylpropanoids linking with sucrose (lapathoside A, lapathoside D) and phytosterols (?-sitosterol, and daucosterol). An HPLC quantification method of the major component of F. esculentum leaves, rutin, was also developed. The optimized condition was performed on a Shimadzu LC-20D HPLC system; Shim-pack GIST C18 column (4.6 mm × 250 mm × 5 µm); eluting solvent system (0-20 min:16-26% acetonitrile in water containing 0.1% formic acid); injection volume 20 ?L; flow rate 1.2 mL/ min; column temperature 35°C; UV wavelength 254 nm. The method was also validated by various parameters, including linearity (R2= 0.9995), precision (6 samples; RSD= 1.58%), accuracy (recover rates from 94.4 to 103.9%), limit of detection (LOD= 0.049 ?g/mL), limit of quantification (LOQ= 0.147 ?g/mL). The analytical results showed that the content of rutin in leaf and flower samples were 1.794 ± 0.002% and 1.137 ± 0.002% , respectively.
format	Bài trích
author	Cong Pham-Van Thi-Ngoc-Oanh Le Thi-Ngoc-Bich Tran Khanh-Linh Nguyen Nhien Le-Thi Tung Nguyen-Huu Hieu Nguyen-Ngoc
author_facet	Cong Pham-Van Thi-Ngoc-Oanh Le Thi-Ngoc-Bich Tran Khanh-Linh Nguyen Nhien Le-Thi Tung Nguyen-Huu Hieu Nguyen-Ngoc
author_sort	Cong Pham-Van
title	Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum
title_short	Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum
title_full	Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum
title_fullStr	Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum
title_full_unstemmed	Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum
title_sort	phytochemical and analytical characterization of constituents from fagopyrum esculentum
publisher	Tropical Journal of Natural Product Research
publishDate	2021
url	https://www.tjnpr.org/viewarticle.aspx?articleid=1721 https://dlib.phenikaa-uni.edu.vn/handle/PNK/2840 http://www.doi.org/10.26538/tjnpr/v5i7.17
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score	8.891787

Phytochemical and Analytical Characterization of Constituents from Fagopyrum esculentum

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