Staff View: Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae

Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae

GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studi...

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Main Authors:	T. T. N. Trinh, A. Gaubert, P. Melani, C. Cambillau, A. Roussel, P. Leone
Format:	Bài trích
Language:	eng
Published:	Acta Crystallographica Section F 2021
Subjects:	camelid nanobodies type IX secretion system Flavobacterium johnsoniae
Online Access:	https://scripts.iucr.org/cgi-bin/paper?S2053230X21005185 https://dlib.phenikaa-uni.edu.vn/handle/PNK/2816 https://doi.org/10.1107/S2053230X21005185
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spelling	oai:localhost:PNK-28162022-08-17T05:54:48Z Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae T. T. N. Trinh A. Gaubert P. Melani C. Cambillau A. Roussel P. Leone camelid nanobodies type IX secretion system Flavobacterium johnsoniae GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studies of GldL to gain information on the assembly and function of the T9SS, two camelid nanobodies were selected, produced and purified. Their interaction with the cytoplasmic domain of GldL was characterized and their crystal structures were solved. These nanobodies will be used as crystallization chaperones to help in the crystallization of the cytoplasmic domain of GldL and could also help to solve the structure of the complex using molecular replacement. 2021-09-13T04:24:47Z 2021-09-13T04:24:47Z 2021 Bài trích https://scripts.iucr.org/cgi-bin/paper?S2053230X21005185 https://dlib.phenikaa-uni.edu.vn/handle/PNK/2816 https://doi.org/10.1107/S2053230X21005185 eng Acta Crystallographica Section F
institution	Digital Phenikaa
collection	Digital Phenikaa
language	eng
topic	camelid nanobodies type IX secretion system Flavobacterium johnsoniae
spellingShingle	camelid nanobodies type IX secretion system Flavobacterium johnsoniae T. T. N. Trinh A. Gaubert P. Melani C. Cambillau A. Roussel P. Leone Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae
description	GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studies of GldL to gain information on the assembly and function of the T9SS, two camelid nanobodies were selected, produced and purified. Their interaction with the cytoplasmic domain of GldL was characterized and their crystal structures were solved. These nanobodies will be used as crystallization chaperones to help in the crystallization of the cytoplasmic domain of GldL and could also help to solve the structure of the complex using molecular replacement.
format	Bài trích
author	T. T. N. Trinh A. Gaubert P. Melani C. Cambillau A. Roussel P. Leone
author_facet	T. T. N. Trinh A. Gaubert P. Melani C. Cambillau A. Roussel P. Leone
author_sort	T. T. N. Trinh
title	Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae
title_short	Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae
title_full	Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae
title_fullStr	Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae
title_full_unstemmed	Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae
title_sort	crystal structures of two camelid nanobodies raised against gldl, a component of the type ix secretion system from flavobacterium johnsoniae
publisher	Acta Crystallographica Section F
publishDate	2021
url	https://scripts.iucr.org/cgi-bin/paper?S2053230X21005185 https://dlib.phenikaa-uni.edu.vn/handle/PNK/2816 https://doi.org/10.1107/S2053230X21005185
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score	8.891145

Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae

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