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Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam

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Main Author: Tran, Diem Hong
Other Authors: Tran, Hau Thi
Format: Article
Language:English
Published: Transboundary and Emerging Diseases 2021
Online Access:https://onlinelibrary.wiley.com/doi/10.1111/tbed.13879
https://dlib.phenikaa-uni.edu.vn/handle/PNK/1846
https://doi.org/10.1111/tbed.13879
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spelling oai:localhost:PNK-18462022-08-17T05:54:45Z Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam Tran, Diem Hong Tran, Hau Thi Le, Uyen Phuong Vu, Xuan Dang Trinh, Thi Bich Ngoc Do, Hoang Dang Khoa Than, Van Thai Bui, Le Minh Vu, Van Van Nguyen, Thi Lan Phung, Huong Thi Thu Le, Van Phan Q1 African swine fever (ASF) is a highly infectious viral disease with high mortality. The most recent ASF outbreak in Vietnam began in 2019, posing a threat to spread to the neighbouring Asian countries. Without a commercial vaccine or efficient chemotherapeutics, rapid diagnosis and necessary biosecurity procedures are required to control the disease. While the diagnostic method of ASF recommended by the World Organization of Animal Health is real-time PCR, the ideal diagnosis procedure including master mix setup, template extraction and a high-cost qPCR equipment for many samples being tested simultaneously is not portable. In this study, a colorimetric loop-mediated isothermal amplification (LAMP) assay was modified and evaluated for ASF virus detection using crude serum samples collected from domestic pigs in Vietnam during the 2019 outbreak. The LAMP results can be readily visualized to the naked eye within 30 min without the requirement of DNA extraction and sophisticated equipment. The sensitivity, specificity and limit of detection of direct colorimetric LAMP assay were comparable to a commercial diagnostic real-time PCR kit. Results strongly indicate that the adapted colorimetric LAMP assay has a remarkable potential for the in-field diagnosis of ASF. 2021-06-17T09:31:48Z 2021-06-17T09:31:48Z 2021 Article Working Paper https://onlinelibrary.wiley.com/doi/10.1111/tbed.13879 https://dlib.phenikaa-uni.edu.vn/handle/PNK/1846 https://doi.org/10.1111/tbed.13879 en Transboundary and Emerging Diseases
institution Digital Phenikaa
collection Digital Phenikaa
language English
description Q1
author2 Tran, Hau Thi
author_facet Tran, Hau Thi
Tran, Diem Hong
format Article
author Tran, Diem Hong
spellingShingle Tran, Diem Hong
Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam
author_sort Tran, Diem Hong
title Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam
title_short Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam
title_full Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam
title_fullStr Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam
title_full_unstemmed Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam
title_sort direct colorimetric lamp assay for rapid detection of african swine fever virus: a validation study during an outbreak in vietnam
publisher Transboundary and Emerging Diseases
publishDate 2021
url https://onlinelibrary.wiley.com/doi/10.1111/tbed.13879
https://dlib.phenikaa-uni.edu.vn/handle/PNK/1846
https://doi.org/10.1111/tbed.13879
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score 8.881002

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