Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam
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Transboundary and Emerging Diseases
2021
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Online Access: | https://onlinelibrary.wiley.com/doi/10.1111/tbed.13879 https://dlib.phenikaa-uni.edu.vn/handle/PNK/1846 https://doi.org/10.1111/tbed.13879 |
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oai:localhost:PNK-18462022-08-17T05:54:45Z Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam Tran, Diem Hong Tran, Hau Thi Le, Uyen Phuong Vu, Xuan Dang Trinh, Thi Bich Ngoc Do, Hoang Dang Khoa Than, Van Thai Bui, Le Minh Vu, Van Van Nguyen, Thi Lan Phung, Huong Thi Thu Le, Van Phan Q1 African swine fever (ASF) is a highly infectious viral disease with high mortality. The most recent ASF outbreak in Vietnam began in 2019, posing a threat to spread to the neighbouring Asian countries. Without a commercial vaccine or efficient chemotherapeutics, rapid diagnosis and necessary biosecurity procedures are required to control the disease. While the diagnostic method of ASF recommended by the World Organization of Animal Health is real-time PCR, the ideal diagnosis procedure including master mix setup, template extraction and a high-cost qPCR equipment for many samples being tested simultaneously is not portable. In this study, a colorimetric loop-mediated isothermal amplification (LAMP) assay was modified and evaluated for ASF virus detection using crude serum samples collected from domestic pigs in Vietnam during the 2019 outbreak. The LAMP results can be readily visualized to the naked eye within 30 min without the requirement of DNA extraction and sophisticated equipment. The sensitivity, specificity and limit of detection of direct colorimetric LAMP assay were comparable to a commercial diagnostic real-time PCR kit. Results strongly indicate that the adapted colorimetric LAMP assay has a remarkable potential for the in-field diagnosis of ASF. 2021-06-17T09:31:48Z 2021-06-17T09:31:48Z 2021 Article Working Paper https://onlinelibrary.wiley.com/doi/10.1111/tbed.13879 https://dlib.phenikaa-uni.edu.vn/handle/PNK/1846 https://doi.org/10.1111/tbed.13879 en Transboundary and Emerging Diseases |
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Tran, Hau Thi |
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Tran, Hau Thi Tran, Diem Hong |
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Article |
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Tran, Diem Hong |
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Tran, Diem Hong Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam |
author_sort |
Tran, Diem Hong |
title |
Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam |
title_short |
Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam |
title_full |
Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam |
title_fullStr |
Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam |
title_full_unstemmed |
Direct colorimetric LAMP assay for rapid detection of African swine fever virus: A validation study during an outbreak in Vietnam |
title_sort |
direct colorimetric lamp assay for rapid detection of african swine fever virus: a validation study during an outbreak in vietnam |
publisher |
Transboundary and Emerging Diseases |
publishDate |
2021 |
url |
https://onlinelibrary.wiley.com/doi/10.1111/tbed.13879 https://dlib.phenikaa-uni.edu.vn/handle/PNK/1846 https://doi.org/10.1111/tbed.13879 |
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1751856253351493632 |
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8.891145 |